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The procedure was as follows: equilibration of the affinity gel as a batch using a glass filter funnel with binding/washing buffer consisting of 10 mM TrisHCl pH 7.8, 0.2 M NaCl, 0.2 mM dithiothreitol (DTT) and 1 mM EDTA

Ren, L., Qi, K., Zhang, L., Bai, Z., Ren, C., Xu, X., Zhang, Z., & Li, X

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Mahapatra, E

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They observed that the structural relaxation of hydrogen bonds established between the DFS and the interfacial water molecules is faster than the hydrogen bonds established between the HFS and the interfacial water molecules