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Furthermore, these metabolites affect MAPK pathways (p38/JNK) and increase epithelial tight-junction expression (occludin, ZO-1, and claudins), strengthening the gut barrier and lowering endotoxin (LPS) translocation

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This linear fragment contained the tag of choice (3x HA for COWP1-HA and HAP2-HA parasite lines) or loxP-flanked recodonised genetic regions and split Cre recombinase (DiCre) genes (inducible GR -KO line), as well as genes for neomycin resistance and nanoluciferase expression, flanked by 50 bp regions of homology to the cut site

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