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The beads were washed with wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP)

Vitamin B12, also known as cobalamin, is a vital nutrient that plays a crucial role in various bodily functions, including red blood cell formation, neurological health, and energy metabolism

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Think of it like: Stabilizing the job site before repairs begin This may allow the body to heal more effectivelybut it still has to do the work

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30 Apart from these, efflux pumps are also a potential mechanism for antibiotic resistance