These findings suggest that tirzepatide has a positive effect on reducing fat in the liver and the amount of fat in the belly compared to insulin degludec
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These approaches have led to significant weight loss with the dual GLP-1/glucagon receptor agonist survodutide (-13.5% with the 6-mg dose at 76 weeks in a phase 3 SYNCHRONIZE-1 trial) [16] , the dual GLP-1 receptor agonist and GIP antagonist Maridebart cafraglutide (known as MariTide) (-12.3% to -16.2% across different doses at 52 weeks in a phase 2 trial) [17] or the triple GLP-1/GIP/glucagon receptor agonist retatrutide (-24.2% with the 12-mg dose at 48 weeks in a phase 2 trial) [18]
For GTT, injection volume was calculated as mentioned below: Food intake Mice were singly housed before the experiment in a 12 h light/dark cycle with ad libitum access to water and were fasted overnight for 12 h (9 pm9 am)
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For in vitro experiments, Dulbeccos modified Eagles medium (DMEM), phosphate-buffered saline (PBS), and Hanks balanced salt solution (HBSS) were purchased from Corning Inc