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TNF, tumor necrosis factor

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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

Journal Biol

This allows researchers to isolate metabolic signaling effects without confounding endocrine responses, making AOD 9604 a useful model for studying localized metabolic regulation