Embryos were then washed twice with 0.1% Tween-20 (Sigma-Aldrich Co., Llc.) in PBS
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Some people might face minor issues, while others could deal with serious problems
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This pattern is different from other types of RCD that have been previously observed
The GSTF8 gene was also induced by H 2 O 2 through the activation of MPK3/MPK6 kinases (Kovtun et al., 2000) the promoter of which contains an as-1 motif, which is implicated in response to oxidative stress (Garretn et al., 2002)