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562 pyridostigmine, an acetylcholinesterase inhibitor that augments sympathetic ganglion transmission to increase vascular resistance during standing without significantly affecting supine blood pressure

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Briefly, the islets were incubated in RPMI 1640 Medium (Gibco) at 37 C overnight, before they were washed in Krebs-Ringer HEPES (KRH) solution with 2.8 mM glucose (low-glucose buffer)

Nucleic Acids Res 47:506515