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To improve the pharmacological properties and enable tumor-selectivity, the phenylazopyridine ligand was terminally functionalized with a hydroxy group that could be used for further chemical modifications

22,28 Given the new evidence that indicates key metabolism enzymes of the folate system are capable of responding to UV and UV-induced damage, it has been hypothesized that functionality of the DNA repair complex will reflect UV exposure

Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)

The findings revealed that this fraction significantly enhanced diuretic and natriuretic effects while maintaining potassium levels