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We separated the growth media from cells grown at a fixed temperature by flowing the liquid cultures through 0.45m-pore filters as previously described

40 Preclinical studies have shown that these effects are mainly mediated trough the effects of GLP-1R activation in the central nervous system (CNS) by the modulation of orexigenic and anorexigenic signaling pathways

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10.1167/iovs.07-0770 57 GorimanipalliB.ShettyR.SethuS.KhamarP

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These cryoEM studies did not capture dynamic coregulator/cofactor-dependent changes in ARs binding surface and context-specific AR function that is consistent with the allosteric regulation of NRs by ligands and coregulators [77, 78, 80, 95, 96]