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Larsen ., 2010, have reported that one of the major causes for sepsis is the increasing levels of free heme released due to hemolysis

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The cecal tissue was immediately frozen with dry ice and stored at 80 C

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Other limitations include the fact that the in vitro granuloma model used is not an exact replication of in vivo granulomas which have a more complex and dynamic environment containing other cells like fibroblasts, collagen matrix, and other white blood cells