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The glutathione-bound toxic substance can then be excreted in the urine, sweat, or feces.[ref] Glutathione S-transferase (GSTM1, GSTP1, etc) detoxification reactions Related article : GST Genetic Variants What depletes your glutathione levels
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Reconstitute the 5 mg vial with 2 mL bacteriostatic water to create a 2,500 mcg/mL concentration
At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid
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