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Follow-up blood work is the clinical gold standard, and we typically re-test after a set number of injections to confirm your levels are rising into a healthy range

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Discussion Significance of the study To our knowledge, this is the most comprehensive network meta-analysis comparing the efficacy of SL, IM, and oral routes of administration of vitamin B12 in patients with vitamin B12 deficiency

Last reviewed: May 12, 2026 Last updated: May 12, 2026 Written by: Jay Hastings , CEO of PlexusDx Jay Hastings is the CEO of PlexusDx, a precision health company focused on genetic testing, blood biomarker insights, and personalized wellness recommendations

Research context Community subcutaneous planning Amount or duration 300500 mcg per day Evidence level Anecdotal community standard Research context Published human trials Amount or duration Route and dose varied by study Evidence level Published research Research context Approved medical dosage Amount or duration None Evidence level AOD-9604 is not FDA-approved AOD-9604 Reconstitution Guide: How to Mix a 5 mg Vial A 5 mg AOD-9604 vial can be mixed with different amounts of bacteriostatic water