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The solution was centrifuged at 1,000 g for 5 min, and the absorbance of the supernatant was measured at 280 nm to determine the lignin content

Int J Endocrinol 2015;2015:390185PubMedPMC 42

Symptoms of Carnitine Deficiency in Dogs The symptoms of carnitine deficiency in dogs are often gradual in onset and can be subtle in the early stages