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If you follow a vegetarian or vegan diet, you may have to seek alternative sources of B12 like tablets or the B12 injection to ensure you have the required levels of B12 to prevent deficiency
The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
A drawback of this assay is that the protein of interest is attached to GST, altering its native state
Nutritional deficiencies may also cause low energy, but supplementation may help increase your energy and ability to exercise, further enhancing your motivation to lose weight
Photochem Photobiol Sci
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