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The beads were washed with wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP)

Current human data therefore cannot attribute fatigue, cold intolerance, or brain fog to altered T4-to-T3 conversion or validate a Free-T3-only monitoring rule. The obese trial cohorts lost roughly 8% of body weight at 1 mg in Phase 2 and about 28% at 12 mg in the 80-week Phase 3 trial

All post-dose glucose levels remained within the normal reference range for the laboratory, and no adverse events of hypoglycemia were reported

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Some adopt a 5 days on, 2 days off weekly schedule as an alternative to full breaks