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According to the instructions of the ATP detection kit (APPLYGEN, Beijing, China), a standard solution and ATP detection working solution were prepared, and the ATP content in the supernatant of the samples was calculated using the standard curve drawn by the standard solution according to the results obtained from the microplate reader

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Besides, our study found that TXNDC12 regulates PAAD ferroptosis via GSH, and since we proved that TXNDC12 inhibits PAAD ferroptosis, we hypothesised that TXNDC12 may influence ferroptosis via GSH