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Promoter reporters and dual-luciferase assay For TGF-1-induced transcriptional reporter assays, HEK293T cells were transfected with one of the SBE4-Luc reporter plasmids

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2.2 Animal studies and antioxidant exposures We utilized male C57BL/6J strain mice (RRID:IMSR_JAX:000664) obtained from a breeder nucleus at the Texas A&M Institute for Genomic Medicine

TMAO influences the bile acid pool by downregulating the expression of key bile acid synthesis enzymes CYP7A1 and CYP27A1