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The growth medium was removed after treatment, and the cells were cultured in 1x MTT (Keygen Biotech, catalog KGA311) in fresh growth medium for 4 h

Oxidative stress status in adults with nephrotic syndrome

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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

First, the causal direction in human studies remains ambiguous: it is unclear whether gut dysbiosis is a driver of OA or a consequence of its associated factors, such as reduced physical activity, diet changes, or chronic medication use