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Retrieved 4 May 2015 via www.neurology.org
GLP-1R Total Internal Reflection Fluorescence (TIRF)-single particle tracking (SPT) INS-1 832/3 GLP-1R KO cells were transiently transfected with WT or V229A human GLP-1R-monomeric EGFP fusion constructs, generated in house by mutating EGFP Ala207 to Lys in hGLP-1R-EGFP [kind gift from Dr Alessandro Bisello (86)] using the following primers: Forward (5 to 3): CCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCA and Reverse (5 to 3): TGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGG, and WT hGLP-1R-mEGFP to V229A with the primers from Table 2
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