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It is also worth being explicit about what documented means here, because it is the load-bearing word for a YMYL page

Each PCR was performed in a 20 l reaction mixture containing 2X KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems)

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Patience and consistent application are truly important for success

furthermore, the activity of reGSTA2X kept more than 60% when it was incubated at 25 to 35 C for 30 min, while incubating the enzymes at 40 C for more than 30 min resulted in 50% activity loss (Fig