Each qPCR run performed in triplicate and negative controls (no cDNA) were conducted
More specifically, the reactive oxidative species (ROS) affect microenvironments associated with the fluid surrounding the egg, fallopian tube fluid, and abdominal fluid and have a direct bearing on egg quality (mitochondria & DNA), sperm-egg interaction, sperm-mediated egg activation, implantation, and early embryo development
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