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Also, salvianolic acid B pretreatment induces Nrf2 and phase II enzymes by activating phosphoinositide 3-kinase (PI3K) and protein kinase C (PKC) pathways, thereby preventing acetaminophen-induced hepatotoxicity in mice (Lin et al., 2015)

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The immunoreactive proteins were visualised using horseradish peroxidase-coupled secondary antibodies (Thermo Scientific) and enhanced chemiluminescence reagents according to the manufacturer's instructions (ECL Select Western Blotting Detection Reagent RPN 2235, GE Healthcare, Amersham)

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