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Cells including at least 3 nuclei and bands of F-actin containing podosomes were defined as mature osteoclasts, and finally mature osteoclasts were counted including the number of per well, the nuclei of each mature osteoclast, and the sizes of the osteoclasts were obtained by measuring the diameters of multinucleated TRAP-positive cells using Olympus IX71 inverted wide-field fluorescence microscope

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Metabolism of nitroglycerin by smooth muscle cells