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Thirty-five-week-old MC4R mice were randomly divided into four groups with no blinding at one day before drug treatment, vehicle (saline, s.c.), semaglutide (0.5 mg/kg, s.c.), tirzepatide (0.05 mg/kg, s.c.), and retatrutide (0.05 mg/kg, s.c.) based on amounts of food intake from day 25 before drug treatment, body weight at day 2 before treatment, and fat mass at one day before treatment

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We found that these mutants do not have centrosome-localizing properties and that one of them loses TuRC-binding activity (unpublished data), suggesting that the mutations cause defects in TuRC-targeting to the centrosome

Potential renal stress markers reported in rodent studies at higher doses

Obesity and aging diminish sirtuin 1 (SIRT1)-mediated deacetylation of SIRT3, leading to hyperacetylation and decreased activity and stability of SIRT3

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