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Given the increasing number of patients with pigmentation secondary to: Hair dye Perfumes and cosmetics Medications Viral infections Hormonal changes like menopause Finding faster and more effective ways to manage, reduce, or dilute pigmentation is essential

Effective stress mitigation strategies include optimizing environmental conditions (good water quality and ideal salinity), minimizing handling and stocking density, enhancing nutrition with targeted nutrients and implementing robust disease management and biosecurity measures [13]

Active factor IXa cleaves factor X at an internal arg-ile (R-I) bond leading to its activation to factor Xa

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Then the supernatant was collected for measuring