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10.1016/j.cell.2017.11.048 29 JiaM.QinD.ZhaoC.ChaiL.YuZ.WangW.et al (2020)

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doi: 10.1002/alz.12088 46 BabaeiP

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Cyclic AMP determination To measure intracellular cAMP concentrations, H9c2 cells and NMCMs were pretreated with 3-isobutyl-1-methylxanthine (0.1 mM, IBMX, Sigma-Aldrich) for 30 min and then stimulated with the non-selective MC-R agonist -MSH (0.1 nM) or the MC5-R agonist PG-901 (0.1 nM) for 5, 15, 30, and 60 min

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HPLC tested with a Certificate of Analysis included

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(Totowa, NJ: Humana Press), 101131

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Researchers must comply with all applicable institutional, state, and federal regulations governing the use of research chemicals

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