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A 2018 study in Dermatologic Therapy found that 38% of patients experienced rebound hyperpigmentation within 812 weeks of discontinuing hydroquinone monotherapy, likely because melanocytes compensate for reduced tyrosinase activity by upregulating MITF (microphthalmia-associated transcription factor), the master regulator of melanocyte differentiation

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The NSCs-specific growth medium was formulated with DMEM medium (Gibco) with 2% B27 supplements without Vitamin A (Gibco), 1% GlutaMAX (Gibco), 1% penicillin/streptomycin (Gibco), 20 ng/ml bFGF (R&D systems), and 20 ng/ml EGF (R&D systems)

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Cycle length and breaks AOD-9604 protocols require structured cycling to maintain effectiveness and avoid potential receptor downregulation