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Use a fresh sterile syringe for each draw

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HLCs were cultured with Alexa-Flour 488-ac-LDL for 1 hour, followed by immunohistochemistry and DAPI counterstaining [1]

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In support of this data, Dias et al

The 28-day rule United States Pharmacopeia (USP) general guidelines indicate multi-dose vials containing preservatives such as benzyl alcohol may be used for up to 28 days after the initial puncture, provided they are stored properly and free from visible contamination