Membranes were sequentially incubated with primary antibodies (anti-GLP-1 [Santa Cruz, sc-80603, 1:1,000], anti-MPC2 [Proteintech, 20049-1-AP, 1:2000], anti-GAPDH [Cell Signaling, D4C6R-97166, 1:5,000]) overnight at 4 C, followed by HRP-conjugated secondary antibodies (Santa Cruz, sc-2354, 1:5,000) for 1 h at RT
Lippincott, Philadelphia Choi I-Y, Lee S, Denney DR, Lynch SG (2011) Lower levels of glutathione in the brains of secondary progressive multiple sclerosis patients measured by 1H magnetic resonance chemical shift imaging at 3 T
GLP-1 receptor agonists mimic this hormone, binding to GLP-1 receptors throughout the body, particularly in the pancreas, brain, and gastrointestinal tract
Baumann MD, Kang CE, Tator CH, Shoichet MS (2010) Intrathecal delivery of a polymeric nanocomposite hydrogel after spinal cord injury
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Clinics offering B12 shots will typically review your medical history and may run blood tests to tailor your treatment plan