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Embryos at 48 hpf were stained with 0.6 mg/mL of o -dianisidine (Tokyo Chemical Industry Co., Ltd) for 15 min, followed by fixation with 4% paraformaldehyde (Fujifilm Wako Pure Chemical Corporation)

Emily Shiffer is a former digital web producer for Mens Health and Prevention, and is currently a freelance writer specializing in health, nutrition, weight loss, and fitness

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