The filtered eluent containing intestinal cells was subjected to two rounds of centrifugation at 120 g for 3 min each cycle in DMEM containing 2% sorbitol
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Catalytic sites may also be determined by aligning a sequence with the sequence of other serine proteases, particularly chymotrypsin, whose active site has been previously determined (Sigler et al., J
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11 , e006740 (2018)