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A clinical trial of glutathione supplementation

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Abbreviations EVs: Extracellular Vesicles OMVs: Outer Membrane Vesicles FMEVs: Feeding Microorganism-Derived Extracellular Vesicles TEM: Transmission Electron Microscopy DLS: Dynamic Light Scattering NTA: Nanoparticle Tracking Analysis DUC: Differential Ultracentrifugation SEC: Size Exclusion Chromatography PEG: Polyethylene Glycol UF: Ultrafiltration TFF: Tangential Flow Filtration cryo-EM: Cryo-Electron Microscopy LC-MS/MS: Liquid ChromatographyTandem Mass Spectrometry PE: Phosphatidylethanolamine CL: Cardiolipin PG: Phosphatidylglycerol LTA: Lipoteichoic Acid PRRs: Pattern Recognition Receptors TLR2: Toll-Like Receptor 2 MAMPs: Microbial-Associated Molecular Patterns MISEV: Minimal Information for Studies of Extracellular Vesicles ESCRT: Endosomal Sorting Complex Required for Transport LOC: Lab-on-a-Chip QPS: Qualified Presumption of Safety GRAS: Generally Recognized as Safe SCFAs: Short-Chain Fatty Acids ZO-1: Zonula Occludens-1 Nrf2: Nuclear Factor Erythroid 2Related Factor 2 HO-1: Heme Oxygenase-1 MAPK: Mitogen-Activated Protein Kinase JNK: c-Jun N-terminal Kinase ERK: Extracellular Signal-Regulated Kinase NF-B: Nuclear Factor kappa B cAMP: Cyclic Adenosine Monophosphate ROS: Reactive Oxygen Species IL: Interleukin TNF-: Tumor Necrosis Factor-alpha TGF-: Transforming Growth Factor-beta Sirt1: Sirtuin 1 MeCP2: Methyl-CpG Binding Protein 2 BDNF: Brain-Derived Neurotrophic Factor TrkB: Tropomyosin Receptor Kinase B PBMCs: Peripheral Blood Mononuclear Cells OGD/R: Oxygen-Glucose Deprivation/Reperfusion tMCAO: Transient Middle Cerebral Artery Occlusion IS: Ischemic Stroke AD: Atopic Dermatitis or Alzheimers Disease (context-dependent) CRC: Colorectal Cancer HIF-1: Hypoxia-Inducible Factor 1-alpha GLUT1: Glucose Transporter Type 1 LDHA: Lactate Dehydrogenase A PDK2: Pyruvate Dehydrogenase Kinase Isozyme 2 MRP1: Multidrug Resistance-Associated Protein 1 ATP7A/B: Copper-Transporting ATPase A/B STAT3: Signal Transducer and Activator of Transcription 3 iNOS: Inducible Nitric Oxide Synthase Arg-1: Arginase 1 DSS: Dextran Sulfate Sodium APP/PS1: Amyloid Precursor Protein/Presenilin 1 HEK293T: Human Embryonic Kidney 293T cells HT29: Human Colorectal Adenocarcinoma Cell Line References Chen X, Li Q, Xie J, Nie S (2024) Immunomodulatory effects of probiotic-derived extracellular vesicles: opportunities and challenges

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The nasal spray format supports repeat testing and is labeled at approximately 1.1 mg per spray, with a total volume of 10 mL (110 mg total peptide content)