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Ucn2 is a 43 amino acid peptide and is expressed in the mouse hypothalamus in the PVN and ARC
ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37C
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Some people confuse mild bloating or water retention (a temporary digestive response to any new supplement) with actual weight gain
Patients running a 16:8 fasting window who inject MOTS-C first thing in the morning often report the most consistent results, though I want to flag that I havent run a controlled comparison and this is mostly pattern recognition
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