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Primer sequences are provided in Data sheet 1 : Supplementary Table S1 (synthesized by Sangon Biotech Co., Ltd., Shanghai)
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IGF-1 plays a central role in tumor development by stimulating proliferation and inhibiting apoptosis in cancer cells. Pollak, Nature Reviews Cancer Acromegaly-Like Symptoms (Rare) In extremely high or prolonged dosesespecially when stacked with HGHIGF-1 may contribute to: Bone thickening (jaw, forehead, hands) Enlarged soft tissue Headaches or carpal tunnel symptoms These side effects mimic acromegaly , a condition caused by excessive GH/IGF-1 signaling
Once detected, tumors were measured weekly using calipers until they had reached a volume of 2.5 cm 3 in size for a single mass or a total volume of 5 cm 3 for multifocal tumors, at which point mice were euthanized in accordance with approved facility protocols