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Throughout the experiment, animals were maintained under anaesthesia, and artificial cerebrospinal fluid (NaH 2 PO 4 2.0 mM, MgCl 2 1.0 mM, CaCl 2 1.2 mM, KCl 2.7 mM and NaCl 145 mM) was constantly pumped through the microdialysis probe at a rate of 0.5 L/min

Biohacker Protocol (experimental) Dose: 2 5 mg (= 2050 clicks) Duration: 4 6 weeks Frequency: 5 days per week Cycle Interval: 2 3 months off before repeating Goal / Description: Microdose continuous approach in experimental aging models

Journal of Chromatography B, 1235 , 124033

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