Mono- and di-PEGylated species were pooled and concentrated, then diluted 12-fold in 100 mM sodium acetate (pH 4.0) and further purified over a HiTrap Macrocap SP column (GE Healthcare #289508-59) using a 0100% elution gradient of 100 mM sodium acetate, 1.0 M sodium chloride, pH 4.0
PG2, Prostaglandin F2
Capito M, Soares R
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