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This article walks through the timeline in plain terms

Safety Considerations for Repeated Use Repeatedly piercing a vial with a needle introduces a small risk of bacterial contamination each time, regardless of which water is used

GSH depletion may result from environmental chemicals and toxins, infections, inflammation, poor nutrition and ageing - compromising the body's ability to defend against cellular damage

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All of this activity requires a significant amount of energy and oxygen, which naturally leads to the production of reactive oxygen species, or free radicals