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Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions

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Whereas initial studies suggest that its expression in the proximal tubules is very low, recent studies using microdissected, freshly-isolated, murine, nephronic segments demonstrated the highest IGF-1R mRNA expression in proximal tubules along the nephron [11]

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In the Richie et al