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Samples were immunoprecipitated with indicated antibodies and protein A/G plus-agarose beads (Santa Cruz Biotechnology) at 4C overnight and washed four times with the lysis buffer
Add 1 mL bacteriostatic water using a fresh insulin syringe inserted at a 45-degree angle against the vial glass wall (not directly onto the powder)
10.1016/j.phytochem.2019.112100 15 BlekkenhorstL
Sinha, K., Das, J., Pal, P
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