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Saturation binding curves were analyzed by GraphPad Prism and fitted to the equation: where B bottom is the fluorescence polarization value of FITC-labeled peptide in absence of protein, B amp is the amplitude of fluorescence polarization signal ( B top B bottom ), X is the concentration of free protein (equal to total protein since [protein][FITC-peptide]), K D is the equilibrium dissociation constant and Y is the fluorescence polarization signal
L., Ball, D
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