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The direct action of GLP-1 which can also be demonstrated in the perfused pancreas, with isolated islets and with beta cell derived cell lines involves activation of the beta cell GLP-1R which couples through the heterotrimeric G-protein Gs to activate adenylyl cyclase and increase intracellular cAMP (27, 65)

human and animal study protocols were approved by the Queens University Belfast (QUB) Faculty Research Ethics Committee or the Animal Welfare and Ethical Review Body

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