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If Grx(SSR) intermediates and mixed disulfides with roGFP2 were more stable and were readily formed, glutaredoxins would sense and transfer a plethora of other redox states to roGFP2 probes

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The degradation rate and half-life (t1/2) of the drugs were determined by measuring the decrease in area under the curve (AUC) at their retention times [2]