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The Framingham Offspring study found that 39% of the general population may be in the low normal + deficient B12 blood level range
Vegetarians are still at risk of developing a B12 deficiency
Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)
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Done incorrectly wrong solvent, harsh mixing, improper storage after reconstitution it can degrade the peptide, introduce aggregation, or invalidate the experiment before it begins
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