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Peptide stacking in the same syringe requires knowing which combinations remain stable
sALS and fALS progression is clinically and pathologically very similar, both characterized by the progressive accumulation of toxic misfolded protein aggregates within cells [76] and the significant loss of MNs [77]
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The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio
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