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However, indirect factors such as nutritional deficiencies from rapid weight loss may contribute to RLS symptoms in some patients
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BPI claims the modification was already known before Lilly filed its patent
Published online 2008
SNAP/FLAG-hGLP-1R serine phosphorylation analysis by immunoprecipitation INS-1 832/3 GLP-1R KO cells were seeded in 6-well plates 24 hours before transfection with 2 g WT or phospho-site mutant SNAP/FLAG-hGLP-1R constructs using Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers instructions, with all subsequent transfections in the study also performed with this reagent
Peiffer, A
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