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Peer review Peer review information Nature Metabolism thanks Alice Adriaenssens, Nigel Irwin and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Two of the proteins of the NOX2 complex are integral membrane proteins and they are identified as p22-PHOX (p22 phox

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Prepare Diluent: Pull an amount of air into the mixing syringe equal to the amount of diluent you plan to add (for EXAMPLE: 1 mL) to create negative pressure