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GLP-1 secretion is enhanced by consuming slow-digesting nutrients like fat, protein, and fiber

For PLA, HEK293, and HEK293 overexpressing HA-tagged human PDGFR (HEK293-PDGFR-HA, a kind gift from Frank Bhmer 109,110 ) were cultured in DMEM and Nutrient Mixture F-12 (1:1) (Gibco) supplemented with 10% (v/v) FBS (Gibco) and PEST

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doi: 10.1210/en.2011-0174 190 HayesMRDe JongheBCKanoskiSE

In research settings, this allows investigators to observe discrete signaling events and timing-dependent assay responses in controlled experimental models