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Retinal organoids (ROs), derived from human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs), are self-forming organoids that mimic the structure and function of native retinas [310, 311]
For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT
A normalizing version of Fishers transformation was applied to normalize the Pearson correlation to a hypothetical N (0,1) distribution of Z scores [43]
lifelong nutritional supplementation and annual blood monitoring are mandatory, with ongoing bariatric MDT follow-up strongly recommended
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