Int J Antimicrob Agents 39, 346351, doi: 10.1016/j.ijantimicag.2011.12.003 (2012)
Thus, sections were deparaffinized, rehydrated and subjected to antigen retrieval with citrate buffer, pH 6, containing 0.05% (vol/vol) Tween-20 (Sigma-Aldrich, Darmstadt, Germany), cooled in distilled water and permeabilized in 0.4% (vol/vol) Triton-X-100 in phosphate-buffered saline (PBS), pH 7.4, on ice for 30 min
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