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This resulted in a dramatic reduction of the high-energy barriers previously obtained with DFT or QM/MM methods, often to zero, enabling the study of vitamin B12 cofactors in in vivo processes
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Similarly, an in vitro study (Nan et al., 2014) using bovine mammary cells demonstrated that Lys, Met, and a combination of these amino acids (especially in a 3:1 Lys: Met ratio) positively regulated the expression of genes associated with the JAK2-STAT5 and mTOR pathway, and increased casein concentration, suggesting a potential effect on enhancing milk protein synthesis
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